Therefore, it is also possible to modify our algorithm to study the coupling mechanisms between exocytosis and endocytosis in the future. Combinatorial microscopy. Google Scholar Employing FM fluorescence imaging, basolateral exocytosis was observed in dispersed rat pancreatic acini after supramaximal CCK or carbachol stimulation; and more remarkably, also after treatment with clinically-relevant concentrations of alcohol and putative alcohol metabolites followed by physiologic CCK or carbachol stimulation 8, 9, 28thus simulating alcoholic pancreatitis. AIP Conf. In summary, we have developed a software platform for the identification and spatiotemporal analysis of vesicle fusion events obtained by TIRF microscopy.
Subhankar Dolai (1,3), Tao Liang(1,3), Laura I.
Cosen-Binker (1). Live pancreatic acinar imaging of exocytosis using syncollin-pHluorin. Building the tracks for exocytotic vesicles: cytoskeleton in exocytosis and cell polarization V.
Manysidedness of a plant cell: delimiting.
Tao Liang at University of Toronto · Tao Liang In this report, a novel live acinar exocytosis imaging technique is described. An adenovirus.
EMBO J. Considering these findings, we conclude that the vesicle exocytosis occurs at spatially preferred regions in INS-1 cells hotspots and that this preference is dependent on the presence of an intact cellular cytoskeleton network.
For example, acidic orange has been found to localize in the acidic compartment not colocalized with granules 7and extracellular labeling cells with fluorescence dextrans cannot distinguish between exocytosis and endocytosis.
The phenomenon, called sequential exocytosis, has been found in many other cells 35— Overall, average fusion rates were reduced to one-third in the CytoD-treated cells and one-sixth in the Nocod-treated cells, respectively Fig. References 1.
10 KILLYNURE AVENUE CARRYDUFF GARDEN
|Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly. Clostridial neurotoxins: new tools for dissecting exocytosis. D Average decay time for each condition.
A A control cell with all fusion events from a time-lapse series marked on the first frame crosses.
Video: Tao living in exocytosis TAO in Everyday Life
Fusion-pore expansion during syncytium formation is restricted by an actin network.
An adenovirus imaging method offers a superior approach to studying pancreatic acinar exocytosis. Subhankar Dolai, Tao Liang, +10 authors Herbert Y Gaisano.
Specific labeling of secretory vesicle exocytosis can be achieved by tagging the vesicle luminal cargos or vesicular membrane proteins with Tao Xu. INS-1 and 3T3-L1 cells were cultured as previously described (27,28). Live-cell imaging. Exocytosis was quantified using live-cell exocytosis and Ca2+ imaging analyses and based on formation of exocytotic soluble N-ethyl maleimide sensitive factor.
Kozka I. Tsuboi T.
This report 42 was largely ignored until more recently when we elucidated the exocytotic machinery mediating this pathologic fusion event 15thus re-establishing the thinking that this is a major event contributing to pancreatitis We would like to thank Dr. Based on particles tracking and statistical testing of the similarity between candidate events and true fusion events, two other algorithms were proposed, but the rate of false positive events was even higher with noisy images 19, The arrow indicates a green hotspot at the junction of basal and lateral plasma membrane region.